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tao chen  (Addgene inc)


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    Structured Review

    Addgene inc tao chen
    Tao Chen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tao+chen/pREDCas9+(Plasmid+%2371541)/pmc12930497-146-12-14
    Average 94 stars, based on 15 article reviews
    tao chen - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541); the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534), and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978). pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and P lexA - recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Article Title: Tuning evolvability via plasmid copy number and regulatory architecture
    Article Snippet: .. The pREDCas9 plasmid carrying the λ -Red was a gift from Tao Chen (Addgene plasmid #71541) . .. The genome sequence was confirmed via whole genome next generation sequencing (NGS) (Novogene Singapore).

    Construct:

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541); the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534), and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978). pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and P lexA - recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Cloning:

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541); the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534), and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978). pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and P lexA - recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..



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